One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 74°C.
COMPOSITION OF THE TAQ MIX
Taq DNA polymerase is supplied in 2X Taq buffer, 0.4mM dNTPs, 3.2mM MgCl2 and 0.02% bromophenol blue. Taq mix buffer is a proprietary formulation optimized for robust performance in PCR.