Hi,

I attached a protocol for preparing liquid mineral medium for growth of baker's yeast. It can be probably also used for many other yeast species.

Regarding the definitions:

liquid medium: medium containing no agar-agar. It is used for growing microorganisms in batch culture, for example.

basal medium: undefined (or complex) medium because it contains a variety of compounds with the exact composition being unknown (e. g., yeast extract). It is used for general cultivation and maintenance of microorganisms.

minimal medium: minimal media contain just the minimum nutrients absolutely required for colony growth. They are used to grow "wild type" microorganisms.

Best regards!

Thank you for you kind information.. it means a lot for me.. Thank you Sir..

Hi,

I agree with Christian definition of mediums. You cand find the composition of YNB, the key element of the minimal mineral medium, at this link:

http://www.formedium.com/europe/SaccharomycesCerevisiae/SaccharomycesCerevisiae_2.htm

Note that "minimal medium" is rarely used in scientific papers, whereas it is more used "synthetic defined" and "synthetic complete" medium. "Synthetic defined medium" (SD) contains normally YNB as source of elements, including nitrogen, and vitamins and a carbon source, that gives the name to the medium. For example SDD is SD + glucose (dextrose), SDE is SD plus ethanol. As said by Christian, only prototrophic strains can grow on it. Synthetic complete medium (SC) is a synthetic SC medium supplemented with all the aminoacids plus adenine and uracil, or drop in mixture (DI), plus a carbon source. All auxotrophic strains can grow on it. Since most lab strains are auxotrophic for one ore more amino acids or bases, normally you can grow them on SD medium supplemented with the specific amino acids (or in SC medium). However if your strain has a plasmid with an auxotrophic marker, you have to grow it in SD strain supplemented with the amino acids for which it is auxotrophic except for the amino acid which is needed for the maintenance the plasmid. As an alternative, especially for slow-growing strains, you can use SD medium supplemented with drop out (DO) mixture, which contains all the amino acids and bases except the amino acid which is needed for the maintenance the plasmid. For example DO-ura contains all the amino acids and bases except for uracil, DO-ade-trp all except adenine and thryptophan.