On google you can see many examples of bone stained with Masson trichrome:

https://www.google.at/search?q=masson+trichrome+bone&biw=1366&bih=599&source=lnms&tbm=isch&sa=X&ved=0CAYQ_AUoAWoVChMIyvmhnYaexwIVA74UCh26twAg#imgrc=_

Collagenfibers are stained by anilinblue.

thanks madam for your link, upon going through various papers, staining of bone trabeculae is varying from paper to paper, in some it is stained red, in some it is stained blue, what is the expected colour?

 The original description of Masson's Trichrome Stain was published in 1929 - In this method collagen was stained with 2.5% aniline blue in 2.5% acetic acid or 2% light green CF in 1% acetic acid (collagen staining blue or green, respectively). Since then Fast Green FCS and wool green S have been also used , giving lighter green stain to collagen fibres. The Google link from Gudrun has both blue and green versions of this stain.  

If you need to intensify a particular part of the trichrome stain: phosphotungstic acid intensifies cytoplasm stain, phosphomolybdic acid - the fibre stain, and alcohol weakens cytoplasm stain. 

You can use a sequence of dyes as in original method or buy a proprietary single solution containing a mixture of dyes. Good luck with your experiments.

Respected Madam, thanks for your response, I am working on Avascular necrosis of femoral head, as part of the project, I am having trichrome stained bone slides, I wanted to know, if its convenient for you, could it be possible for you to interpret images. If it is ok with you then may I have your email ID so that I can pass on the presentation having the stained images for your interpretation, thanking you, with sincere regards aswath phd student sri sathya sai institute of higher learning, email ID: [email protected]

Masson's method stains differently a mineralized bone (blue) and an osteoid (red)

Dear dr Farrag (and the rest of contributors),

It is not the first time I hear such a thing (blue/turquoise is mineralized collagen vs red is osteoid). However, I never found any serious publication backing that statement. Would you have by any chance some reference to provide?. If that would be true, then it wouldnt be necessary to do MMA (plastic) embedding of undecalcified bone to do histomorphometry assessments of diseases related to the accumulation of osteoid (osteomalacia/rickets). Moreover, my own observation of samples do not correspond with that statement, showing in many cases patchy red/blue staining in mineralized bone matrix. I've trying to find a convincing explanation on why this happens, but with no success so far. I've read discussions about it being produced by the compactation of colagen fibers, their polarity based on a higher exposure of the N- or C-terminal end of the molecule, etc etc, but I didn't find a single publication sucessfully addressing this phenomenon.

Thanks!

green