What is the coating on a gelbond film?

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Does anyone remember how to take a FISH image with Metasystems Isis software?

Does anyone remember how to take a FISH image with Metasystems Isis software and then take another at a different focal plane and combine them? I used to do this but now can't remember! Thanks

14 May 2024 10,025 0 View

What is the status of the principle of Justice in our legal system?

Oliver Wendel Holmes believed that justice had to do with customs that vary from one society to another, relativistic and without a foundation as an overarching principle. Marxist believe that...

22 March 2023 4,828 4 View

Contamination in primary bone marrow MSC culture?

Recently I’ve started seeing these clumps in my primary MSC flask (T175). I first noticed these compact clumps in a flask near 80% confluence but I’m also seeing it in less confluent flasks. The...

22 February 2023 9,528 3 View

Best means of quantifying cDNA for qPCR Input?

My lab has been using the Qubit ssDNA kit to quantify cDNA prior to qPCR input, but we have doubts about the accuracy of this kit. The MFR explicitly states that the Qubit ssDNA kit does not bind...

14 December 2022 1,052 2 View

Can we test one-directional hypotheses with ANOVA?

It is said that the p value for a t-test can be calculated as one-tailed or two-tailed. This makes sense as Condition A scores can be higher, or lower than Condition B scores (so a two-directional...

04 March 2022 3,629 4 View

What is the estimated clearance time or half life of LPS in mice, when inhaled?

I have seen some literature discussing clearance of LPS from the bloodstream, where it can be cleared pretty well in about 6 hours (Lipopolysaccharide Is Cleared from the Circulation by Hepato......

05 August 2021 2,263 1 View

What data is collected by Nurses in the Mobile Clinics in South Africa?

Traditionally patients in South Africa, particularly in rural areas retain their own clinical record, often in an exercise book or similar. Healthcare staff also record the history of a particular...

14 May 2021 3,930 3 View

Can artificial splice sites be used to create two transcripts?

Is it possible to have two protein variants transcribed from a transfected plasmid (or viral vector)? Not in the sense of using IRES or P2A/T2A, but rather in having two proteins with the same C...

02 March 2021 7,761 2 View

Can anyone suggest various protocols/stains for culturing and imaging Myxamoebae using a light microscope?

All suggestions welcome. We are curious to find out what works well, and to hear about diverse methodology. Thanks! We currently have restricted access to a lab where Silver staining etc could...

09 June 2020 9,263 3 View

UK Practice MRI Knee : what factors from the radiology report are used in decision making for arthroscopy ?

If an MRI knee scan identifies a meniscal tear; what factors are taken into consideration to send for arthroscopy? If the scan has been generated from a GP referral: is there vetting to decide...

14 May 2020 352 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

What procedure should I follow for current controlled PPy film electrochemical deposition on ITO?

For example, I perform passivation process before electropolymerization of Py for adhesion to titanium surface. Is a similar or different procedure required for ITO? If necessary, I would be glad...

06 August 2024 3,176 0 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

How can I better use imageJ to get good result on calculating surface area of a film accurately?

I have images of film formed on the bottom of a 50mL beaker, and I have been trying to figure out the surface area. I could easily use features on imageJ to do that, but I want to know which area...

23 July 2024 4,461 1 View