I am looking for classical method/SOP (Standart Operation Protocol) to quantify this specific group Nitrosomonas. Any information would be greatly appreciated.
use the MPN method - it is described in LEVIČNIK HOEFFERLE, Špela, NICOL, Graeme, PAL, Levin, HACIN, Janez, PROSSER, James Ivor, MANDIĆ-MULEC, Ines. Ammonium supply rate influences archaeal and bacterial ammonia oxidizers in a wetland soil vertical profile. FEMS microbiol. ecol., 2010, vol. 74, no. 2, str. 302-315