What is the characteristic peak in Circular dichroism spectra for ssDNA?

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Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

How is artificial intelligence being utilized to enhance the diagnosis and treatment of sleep apnea?

AI has the potential to improve the management of sleep apnea by personalizing treatment, enhancing diagnostic accuracy, and advancing our understanding of the condition.

03 July 2024 9,393 2 View

How to dilute my DNA Template( 1 Micromolar stock solution ) into1 × 10-8 nM to 1 × 10-15 nM ?

How can i dilute my master stock of 1 micromolar DNA template to different concentration by serial dilution

17 May 2024 3,641 2 View

Why can't Ansys HFSS be used for LiDAR Based Simulation?

Hey All, I am currently trying to simulate an FMCW Lidar in Ansys HFSS which I used for RADAR Simulation and I cannot find a way out to perform Lidar based simulation. If anybody knows how to do...

20 March 2024 2,749 2 View

Hi, A phospholipid qualitative analysis is to be performed in liposomal formultations.Is HPLS-UV a reliable technique for same ?

A phospholipid content has to be estimated in liposomal formulations.Is HPLC-UV a reliable technique for same or should other techniques be employed?Which one is the most suitable of them all ?

22 November 2023 4,883 1 View

How to generate ligand.itp and ligand.gro files for md simulation using amber14 force field?

How to generate ligand.itp and ligand.gro files for md simulation using amber14 force field?? Kindly help!! Thanks in advance.

12 November 2023 2,434 4 View

Where I can do extrusion of Magnesium alloy in india?

Any vendor or Institute.

05 November 2023 3,030 0 View

Error while generating ligand topology?

I'm trying to generate ligand topology files through CHARMM FF but i'm getting this error. I installed the respective python version but error is still same python cgenff_charmm2gmx.py UNK...

23 October 2023 1,587 4 View

How can I insert a protein structure model within Lipid bilayer structure model?

How can I insert a protein structure model within the Lipid bilayer structure model? Kindly help.

12 May 2023 5,082 3 View

What are the alternate servers for PRODRG to generate ligand topology? I have tried ATB, ACPYPE also but ligand gets detached from the protein?

what are the alternate servers for PRODRG to generate ligand topology? I have tried ATB, ACPYPE also but ligand gets detached from the protein while generation complex.gro file in the MD run....

23 April 2023 7,765 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

Why do some of my Raman spectra show no signal at lower Raman shifts?

I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...

30 July 2024 8,771 3 View