Is setting an apparently impossible goal the best way to make a real breakthrough in science?

10 November 2017 5,021 72 View

Which technologies will influence and shape the science and society at the most in near future?

Technology has been closely associated with the evolution of civilization starting with tools, clothing, fire, shelter, natural resources conversion and various other things of basic survival....

04 May 2017 1,701 89 View

Universal Quality Basic Education: where are we lagging behind and what are the ways ahead?

04 May 2015 4,555 11 View

What are the potential unintended consequences of Nanotechnology to human health and environment?

Nanotechnology has attracted the attention of researchers across the world and until today it is recognized as one of the most important fields of scientific endeavour. Many unique properties and...

01 February 2015 9,504 54 View

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Our contemporary health care systems are generally directed towards bringing ailing people out of illness. This takes sick from negative health to a state that is neutral or free from health...

31 December 2014 9,889 59 View

What steps can we take to improve our ability to tackle challenges like climate change that cross national boundaries?

08 September 2014 1,394 29 View

As a science-minded person, what makes you optimistic and why?

Science is fundamentally optimistic; per se, a science minded (not only designated Researchers) ought to have confidence in optimism. While conventional perception tells us that things are bad and...

08 September 2014 3,631 44 View

What is your opinion about Obligatory Authorship in research publications?

Owing to substantial increase in the collaborative and multi-centric research, authorship issues have arisen in the recent years. Authorship disputes account for 2-11% of all disagreements in the...

07 August 2014 9,626 72 View

What do you think about the prospects of new emerging technologies and how these new domains will tackle the social & ethical issues?

Nanotechnology, Biotechnology and Information & Communication Technology are associated with contemporary domains of new S&T development. These emerging technologies though mostly in early...

07 August 2014 3,680 32 View

How do we meet food and energy requirements of our future generations?

06 July 2014 496 52 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

A good day to you, Great minds. How do I evaporate hexane and Ethyl acetate extract of bacterial metabolite?

I'm really struggling with it. It takes hours on a water bath at 50 degrees

03 February 2021 1,619 3 View

How can I measure speed of integration of an acquisition with secondary data?

I am currently faced with the challenge to measure integration speed of an acquisition or a merger with secondary data. Usually companies do not communicate once a target has been integrated...

27 January 2021 9,741 11 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View