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Questions related from Samuel John Prendergast
If I isolate/enrich lysosomes from mammalian cells through either differential centrifugation or using commercial kits, what is the best method of storage to preserve enzymatic activity? Is there...
30 June 2021 8,654 2 View
I am hoping to perform TEM on exosomes enriched/isolated by ultracentrifugation from cell lines. In an effort to save time and money during optimization; can anybody suggest a good concentration...
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I am hoping to optimise my exosomes yield when ultracentrifuging conditioned media of cultured cells. Protocol so far is: 30 min at 2,000x g (remove cells and large debris) 60 min at 10,000 x g...
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I realise this differs between cell lines so an exact answer is not possibly. But if anybody could tell me the cells they work with and the exosome protein quantity they achieve it would help me...
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