Hello, could anyone please suggest some of the best ways to stain specifically mouse glomeruli for confocal microscopy?
Hi, I am working on heat stress in dairy cows. In a recent experiment, we used a FLIR-T62101 thermal camera to take photos of the cows. One photo is attached. How can I analyze these images to...
30 June 2024 2,190 5 View
Is it acceptable to include information or findings that have not been formally published (i.e., not yet shared in scientific journals) in a review article. Whether the unpublished information...
21 February 2024 2,769 4 View
When I try to add my academic email, the Research Gate website says they sent a verification email. But I received no such email in my academic mail. I checked spam and all other locations. Help...
07 January 2024 3,587 1 View
2D material can be defined as a user defined material in silvaco, but can not be performed the quantum computation . Now if I want to use Silicene as channel material with proper quantum...
15 September 2023 5,461 0 View
I write the code for 3rd derivative of id-vg curve. extract name="dgm2_d2vgs" deriv(v."gate",i."drain",3) outfile="dgm2_d2vgs.dat" now the point of gate voltage where y .val=0 is the threshold...
28 August 2023 6,712 0 View
26 August 2023 2,759 0 View
Hello everyone, I am trying to create a phase shifter like this figure in CST for the beam-steering antenna. Please give me suggestions or references so that I can do this. Thanks
30 December 2022 407 3 View
I am trying to reconstruct microwave breast images using open-source software Merit in Matlab. I have simulated a breast phantom in CST and for reconstructing the image I need to extract some...
15 February 2022 7,421 1 View
I am currently doing my thesis in microwave breast imaging and I have simulated a breast phantom in CST. I have extracted the S21 data in .txt format. Now I want to plot the breast images using...
09 February 2022 5,913 6 View
Please explain with an example, if possible.
28 December 2021 3,600 10 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View