What is the best way to pretreat and swell CM52 cellulose cation exchanger?

Girishkumar Kumaran @Girishkumar-Kumaran

05 June 2017 1 2K Report

I have in lab CM52 cation exchanger from Whatman that I wish to try to use it for purification. However, I could not find any material that speaks about the swelling and pretreatment of CM52. Does it need pretreatment or can I go ahead and equilibrate with buffer and use it?

Or in general, how do we go about swelling and pretreatment of cation exchangers?

Mushtaq Ahmad

http://www.pnas.org/content/82/8/2267.full.pdf

equlibrate in same buffer

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