Girishkumar K. Kumaran

5 Questions 18 Answers 0 Followers

Questions related from Girishkumar K. Kumaran

When doing qPCR, I see my silenced values greater than the control. Could someone please throw some insights into what could be happening?

I am doing qPCR to quantify silencing some of the proteins I work with. but I see that my silenced values are greater than that of the control ones. I got higher values in silenced samples after...

12 October 2022 4,165 7 View

What is the best way to pretreat and swell CM52 cellulose cation exchanger?

I have in lab CM52 cation exchanger from Whatman that I wish to try to use it for purification. However, I could not find any material that speaks about the swelling and pretreatment of CM52. Does...

05 June 2017 1,676 1 View

How do water molecules overcome the van der waals distance and form hydrogen bonds?

I read in Voet & Voet that the hydrogen bonds are at least 0.5 angstrom shorter than the calculated van der waals distance. As I understand, van der waals distance is the distance of closest...

27 May 2017 6,869 4 View

What is the best way to prepare pichia pastoris whole cell lysates for SDS PAGE to check for expression?

I am trying to purify a membrane protein using Pichia pastoris expression system. I wish to know what is the best way to prepare whole cell lysates to check for expression

13 March 2016 4,548 4 View

What is the role of biotin in BMGY media?

I wish to know the role of biotin in BMGY (Buffered Glycerol Complex Medium) media used for Pichia pastoris cultures.

14 February 2016 2,545 5 View