Do you have suggestions for feed formulation software?

Hi, I am considering buying a feed formulation software that could be used for teaching as well as animal experiments for both ruminants and monogastric species. Do you have any suggestions for...

11 December 2018 5,076 34 View

How to calculate area under curve (AUC) in R?

I would appreciate if you share R codes, information, suggestions etc. regarding calculation of area under curve in R for time-series data. Thanks

08 September 2017 2,755 7 View

How to compare histograms from different groups in R?

07 August 2017 6,077 24 View

Independent t-test with covariates?

Hi all, I have two independent groups I want to test whether their values differ significantly for a number of parameters studied. Based on my statistical knowledge from early university, the...

01 February 2017 9,817 13 View

How to use lsmeans to make interaction plots in R?

Hello there, I would appreciate if you could provide some tips on how to use lsmeans to make interaction plots in R. Thanks alot

01 February 2017 3,337 11 View

How to deal with two repeated variables (nested) in lme R?

11 December 2016 2,334 5 View

In a repeated mixed model analysis, the repeated variables such as time or week etc. are treated as a factor or continuous variable in the model?

Hello there, I am performing repeated measures analysis where I need to look at changes in blood parameters over a period of time (lets say week). So, should I use week as a factor or continuous...

09 October 2016 5,615 10 View

What is the best way to measure gluconeogenesis in-vivo in mice?

Would you please let me know what would be the best way to measure gluconeogenesis in-vivo in mice, I would appreciate.

09 October 2016 2,209 2 View

What could the best way to preserve mice pancreas to obtain good RNA yield for qPCR?

Hello everyone, I had had a difficulty in obtaining good RNA yield from sheep pancreas samples in the past. This time I am going to try with mice samples. Would you please suggest effective ways...

08 September 2016 7,513 3 View

Glucose tolerance test in mice: Do you prepare 20% glucose solution in NaCl solution or in water?

I would appreciate if you can answer whether you prepare glucose solution for injection in mice in NaCl solution or just in water and why!

07 August 2016 7,175 9 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View