How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

No title

Hi there! I'm very new to r and trying to run a multi-level meta analysis using pre-calculated effect sizes. I've attached the datafile below. The script I used as a first step to fit the model...

12 January 2021 8,214 2 View

Non parametric alternatives to one-way ANOVA for 4 groups?

Hi, Currently assessing NDVI data (vegetation index, continuous data) against land type (4 groups, categorical variables). My data is not normal, or has equal variance (transforming hasn't...

14 December 2020 7,796 3 View

How to remove the jacketing from the midsection of an optical fibre?

I have a HCS fibre with a 200 micron diameter. I want to remove the jacketing in sections along its length so I can replace the cladding underneath and create an evanescent field sensor. Obviously...

13 December 2020 3,068 2 View

the lack of promotion bringing dissatisfaction on the employees?

Write a report with literature review and recommendations for The relationship between lack of promotion and dissatisfied employees to apply your management role to resolve for employees.

21 October 2020 6,698 2 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

Please help: R problems with forest plots ??

Hello, everyone! I am having trouble running my syntax for my forest plot. Below is the syntax I have used previously, with the changed data frame for my current analysis. For whatever reason, the...

27 June 2020 641 1 View

Does anyone know of a parent-report measure of child loneliness suitable for kids between 6 and 12?

This will be used in a study of children's well-being (we will only have access to parents).

23 March 2020 8,355 3 View

How to calculate substrate consumption rate?

Hello lovely people, It is my first question here, so I hope you can help me.. For an experiment of my thesis I provided pure cultures with different concentrations of lactate an monitored them...

26 February 2020 1,114 3 View

Troubleshooting Cryostat sections?

Hello everyone, I have beginning work in a new lab where I never use the cryostat (but I did it a ton of times in my previous lab). I cut mouse visual cortex at -20 and -18 with sections of 20 um....

24 February 2020 4,202 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View