It depends on the material on which you are working. We have been working on maize where F. verticillioides is as a common endophyte occurring in seeds and at every stage of corn growth. In this case you have to isolate from It is also a pathogen (ear and stalk corn rot) and you can isolate it from symptoms. Moreover, identification at species level may be difficult and you should use different methods, from biochemical to molecular. We add some of our papers on this topic. Consider we have a wide collection of Fusaria. Best regards Gemma Assante

1. Venturini Giovanni, Assante Gemma, Toffolatti Silvia Laura, Vercesi Annamaria (2013). Pathogenicity variation in Fusarium verticillioides populations isolated from maize in northern Italy. Mycoscience 54 (4): 285-290. http://www.sciencedirect.com/science/article/pii/S1340354012000460

2. Venturini Giovanni, Assante Gemma, Toffolatti Silvia Laura, Vercesi Annamaria (2011). Mating behavior of a Northern Italian population of Fusarium verticillioides associated with maize. J Appl Genetics 52:367–370. DOI 10.1007/s13353-011-0042-4. Or http://air.unimi.it/bitstream/2434/159568/2/Venturini_2011JOAG.pdf

3. Venturini Giovanni, Assante Gemma, Vercesi Annamaria (2011). Fusarium verticillioides contamination patterns in Northern Italian maize during the growing season. Phytopathol. Mediterr. (2011) 50, 110−120. http://air.unimi.it/bitstream/2434/157340/2/Phytopathmedit_FUSARIUM2011.pdf

http://www.sciencedirect.com/science/article/pii/S1340354012000460; http://air.unimi.it/bitstream/2434/159568/2/Venturini_2011JOAG.pdf ; http://air.unimi.it/bitstream/2434/157340/2/Phytopathmedit_FUSARIUM2011.pdf

Dear

It depends basically on the materials on which you are doing Research work. We have been working on maize where F. verticillioides is as a common endophyte occurring in seeds and at every stage of corn growth. In this case you have to isolate from It is also a pathogen (ear and stalk corn rot) and you can isolate it from symptoms showing the disease infection. Moreover, identification at species level may be difficult and you should use different keys and methods, from simple cultural and biochemical to molecular techniques. You can see Research papers on the topic. Different isolates behave differently specially on Pathogenicity variation in Fusarium verticillioides populations should be Consider ed. You may send your collections to some Type culture collections for identification please.

Best regards

Ebrahim

Dear

It depends basically on the materials on which you are doing Research work. We have been working on maize where F. verticillioides is as a common endophyte occurring in seeds and at every stage of corn growth. In this case you have to isolate from It is also a pathogen (ear and stalk corn rot) and you can isolate it from symptoms showing the disease infection. Moreover, identification at species level may be difficult and you should use different keys and methods, from simple cultural and biochemical to molecular techniques. You can see Research papers on the topic. Different isolates behave differently specially on Pathogenicity variation in Fusarium verticillioides populations should be Consider ed. You may send your collections to some Type culture collections for identification please.

Best regards

Ebrahim

Dear

It depends basically on the materials on which you are doing Research work. We have been working on maize where F. verticillioides is as a common endophyte occurring in seeds and at every stage of corn growth. In this case you have to isolate from It is also a pathogen (ear and stalk corn rot) and you can isolate it from symptoms showing the disease infection. Moreover, identification at species level may be difficult and you should use different keys and methods, from simple cultural and biochemical to molecular techniques. You can see Research papers on the topic. Different isolates behave differently specially on Pathogenicity variation in Fusarium verticillioides populations should be Consider ed. You may send your collections to some Type culture collections for identification please.

Best regards

Ebrahim

Dear

It depends basically on the materials on which you are doing Research work. We have been working on maize where F. verticillioides is as a common endophyte occurring in seeds and at every stage of corn growth. In this case you have to isolate from It is also a pathogen (ear and stalk corn rot) and you can isolate it from symptoms showing the disease infection. Moreover, identification at species level may be difficult and you should use different keys and methods, from simple cultural and biochemical to molecular techniques. You can see Research papers on the topic. Different isolates behave differently specially on Pathogenicity variation in Fusarium verticillioides populations should be Consider ed. You may send your collections to some Type culture collections for identification please.

Best regards

Ebrahim

Dear

It depends basically on the materials on which you are doing Research work. We have been working on maize where F. verticillioides is as a common endophyte occurring in seeds and at every stage of corn growth. In this case you have to isolate from It is also a pathogen (ear and stalk corn rot) and you can isolate it from symptoms showing the disease infection. Moreover, identification at species level may be difficult and you should use different keys and methods, from simple cultural and biochemical to molecular techniques. You can see Research papers on the topic. Different isolates behave differently specially on Pathogenicity variation in Fusarium verticillioides populations should be Consider ed. You may send your collections to some Type culture collections for identification please.

Best regards

Ebrahim

you can send high resoultion images from your Fusarium spp.to me please

Thanks,

Ebrahim

Dear Dr.Olowe,

We have isolated Fusarium solani from clinical and environmental samples on our Pal sunflower seed medium.We developed this medium in 1980 for rapid isolation and presumptive identification of Cryptococcus neoformans. We have published many papers on Pal sunflower seed medium.You may please try our medium for the isolation of Fusarium verticillioides.

Wishing you best of luck in your scientific career.

Prof.Dr.Mahendra Pal

Founder Director of Narayan Consultancy

Thank you @ Prof.Dr.Mahendra Pal.

The study had already been carried out and published