What is the best way to isolate a specific protein from total cell lysate?

17 October 2023 1 217 Report

I am brand-new to the world of protein research. I have total cell lysate samples that I would like to extract RAB5 and RAB7 proteins from. Which is the best approach to do this?

Sabra Parveen

You can use RIPA lysis buffer protocol for whole cell lysate preparation. There are various protocols available for the same.

https://www.abcam.com/protocols/sample-preparation-for-western-blot

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