We have isolated some bacterial isolates. We have PCR amplified some already reported virulent genes from its genomes. What is the best possible appraoch to look for new /novel virulent genes encoded by these pathogens?
Now a day best way is hole genome sequence, but cheaper way is reviewing other studies in different area and do PCR.
Dear , Sadeeq Rahman
Sequencing of the 16S rRNA gene (16S) is a reference method for bacterial identification. Its expanded use has led to increased recognition of novel bacterial species. In most clinical laboratories, novel species are infrequently encountered, and their pathogenic potential is often difficult to assess.
Best regards
Dear Sadeeq Rahman ..
You can return to the following papers , they may be helpful .
1 - Robert Schlaberg, 1 Keith E. Simmon,1 and Mark A. Fisher.2012. A Systematic Approach for Discovering Novel, Clinically Relevant Bacteria. Emerg Infect Dis. 2012 Mar; 18(3): 422–430.
2 - Sameer A. Barghouthi . 2011 .A Universal Method for the Identification of Bacteria Based on General PCR Primers . Indian J Microbiol. 2011 Oct; 51(4): 430–444.
3 - Kai Shen et al. 2005. Identification, Distribution, and Expression of Novel Genes in 10 Clinical Isolates of Nontypeable Haemophilus influenzae.Infect Immun. 2005 Jun; 73(6): 3479–3491.
With my best wishes
اhi dr. My relationship with my very good With my best wishes
yup, this article is great and it will be in help:
Sameer A. Barghouthi . 2011 .A Universal Method for the Identification of Bacteria Based on General PCR Primers . Indian J Microbiol. 2011 Oct; 51(4): 430–444.
Another possible and simple strategy is to target IS elements and integrons.
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