What is the best way to identify novel virulent genes in a clinical bacteiral isoalte??

We have isolated some bacterial isolates. We have PCR amplified some already reported virulent genes from its genomes. What is the best possible appraoch to look for new /novel virulent genes...

08 September 2017 4,477 6 View

How would you do in vitro immunogencity test for an immunodominant protein??

We have found out a couple of virulent genes that could be potential immunodominant proteins as well from bacteria isoalted from bovine mastitis. We have cloned them, expressed them in bacteria....

08 September 2017 6,078 2 View

Need help in cloning IPTG inducible promoter upstream a gene?

I want to clone IPTG inducible promoter upstream a gene. Is there any advice how can i do it. some people have used rrnB ti terminators upstream to lacOid and lacI gene for tight expression. I...

04 May 2016 1,594 1 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View