What is the difference between master and doctoral degree?

Hello there.. I've recently submitted my master thesis in marketing. As a reseacher, I now know that the purpose of a master degree is to know academic research tools and how to do and write an...

09 February 2021 6,692 9 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How can I write a Scientific proposal to present it in public conference?

Writing Proposal

30 December 2020 6,783 3 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How can i separate a polymer after a polymerization reaction from the unreacted monomers, taking in consideration that it's really sticky?

The polymer i'm fabricating will be used to make a delivery system, so it must be separated from the monomers right ? i tried to use solvent antisolvent method but the polymer is only soluble in...

13 October 2020 2,002 4 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is the reason of introduction of SDM 6 nucleotides before the right position in a gene on a plasmid when sequenced?

I am trying to introduce SDM for a gene on a plasmid. After Pcr (with HF Q5 polymerase and primers designed by NEB website the length of one of them 19) , Dpn 1, ligation, transformation and...

27 September 2020 5,493 11 View

Is Thermodynamics not able to get the heat transfer rate value?

In the first lecture of heat transfer course, generally it is told that with Thermodynamics, it is not possible to get the heat transfer rate of a process. But in steady flow energy equation (time...

29 February 2020 9,700 9 View

What is role of toluene and stearic acid in ball milling for powder ? In what weight percent we should add this to powder?

I am doing ball milling operation of Aluminum and silicon carbide powder. I am pretty confused about what should be added as process control agent ? what is role of toluene as PCA and stearic...

12 February 2021 5,205 3 View

What is the reason for using Glass Impingers?

Impinger bottles are used at some applications, where the gas is cooled and the condensate is dissolved in a solvent contained in the impingers. I have always seen impingers made of glass. Why not...

03 February 2021 6,959 2 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

How can solve/disperse silicon nanoparticles in water?

I want to add silicon nanoparticles to my hydrogel, but when I sonicated these particles with water to disperse them in water, they agglomerated on the water's surface. How can I disperse them in...

26 January 2021 213 4 View

Anyone know how to dilute strong hydrogel structure?

I am working on PVA-GMA hydrogel that is a really strong polymeric structure like rubber. Because I am in the middle of analysis, it is difficult to dissolve the gel using a common solvent. I...

20 January 2021 3,501 2 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

In order to simulate a DWC in steady state, how to put the unit operations in the flowsheet of any process simulator?

14 January 2021 3,938 6 View

Why Selenium nanoparticles synthesized by PEG 200 could not be scaled up ?

I could not synthesize Selenium nanoparticles by PEG 200 at up-scale ( more than 100 ml) as it got aggregated while at small amounts it works fine , I work with 850 PPM using selenium powder in...

08 January 2021 10,049 1 View

Is it reliable to use toluene as a solvent in measuring density by liquid displacement method?

Hi all, I am working on pollen and want to examine the true density. No doubt, toluene does not swell the particles but it may dissolve the lipid materials and other organic molecules besides the...

07 January 2021 2,471 2 View

Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View