Mariam Hakeem-Sanni

3 Questions 2 Answers 0 Followers

Questions related from Mariam Hakeem-Sanni

What is the best way to seed cells on acrylamide coated coverslips?

i am trying to seed cells on coverslip coated with gels (acrylamide based hydrogels) My main obstacle is my cells aren't settling down as expected so they can grow instead they keep floating, or...

02 November 2015 8,863 3 View

What is the best way to dissolve N-Hydroxysuccinimide (NHS) in toluene?

I need to prepare saturated NHS solution, according to the protocol I'm using, I need saturated NHS in toluene to prepare hydrogels and the saturated solution is meant to form a cloudy...

10 September 2015 5,226 1 View

Why does my citrate buffer turn milky during immunohistochemistry?

For my IHC protocol, I am staining for beta-catenin in ovarian sections during antigen retrieval. My samples are heated in citrate buffer for 45 mins. I have also read that signs to show that your...

09 December 2014 2,056 3 View