We would like to estimate total concentration of arsenic, cadmium, and some essential elements such as zinc and iron in the bones and nail collected from rabbit.
Dr Jamwal
please follow the article:
http://article.sapub.org/pdf/10.5923.j.env.20120204.05.pdf
Acid digestion with nitric and perchloric acid (4:1 ration). Go for microwave digestion if have the facility otherwise you can use a hotplate. In a case of hot plate digestion, wet the samples overnight with the acid mixture then reflux at 150-degree using sediment bed.
Dear Dr Jamwal,
Acid digestion without microwave should be sufficient. I attach a few of our articles on this issue. Please use the best analytical grade of the reagents as contamination of the ordinary agent may interfere mainly with trace metals as zinc, copper, selenium.
If you need any more information, please do not hesitate to contact me.
All the best,
Frederik de Wolff
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Dear Mr. Jamwal,
The information provided by Dr. Wolff is very useful. A simple way to perform the acid digestion of these samples is that you may initially digest the finely ground material (0.5 to 1 gm) on sand bath in conc. HNO3 (10 mL) till brown fumes largely stop. This step is necessary as some lipids may be present in bone samples. After cooling of the acid digest solution, add 10 mL of 3 : 1 (v/v) mixture of conc. HNO3 and HClO4 and continue digestion till the volume is reduced to 2 mL or so. care should be taken that the sample is not charred at any stage. Again after cooling, the digest may be treated with 5 mL 6 N HCl. After gentle boiling and cooling the digest may be diluted and filtered. The final volume may be made to 50 mL or so by using distilled water.
Dear Mr. Jamwal,
The use of Concentrated HNO3 as advice by Dr. Wolff can enhance the extraction process.
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