What is the Best Way to Determine Vitamin A Palmitate by HPLC?

02 July 2018 0 4K Report

Hi Everyone,

Sample: Powder Vitamin&Mineral Mixes and Powder Vitamin A Palmitate Samples

Devices:

- Shimadzu High-Pressure Liquid Chromatography System Prominence LC-20A - CBM-20A lite HPLC System Controller - LC-20AT Pump - DGU-20A5R Degassing Unit

- 7725i Manual Injection Unit - CTO-10Asvp Column Oven - SPD-20A, UV/VIS Detector - Inertsil ODS-3 5um 4.6*150mm - InertSustain C18 5um 4.6*150mm

-Rotary Evaporator etc...

Can you suggest best method to apply with this instruments?

I have never used nitrogen gase in lab, how can I adapt/use it to prepare sample for HPLC? I found lots of methods which includes saponification step but ı have no knowledge about this step.

Awaiting for your valuable advises:)

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