How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Is there any difference between "SEP" as a non standard residue and phohophorylated serine?

I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...

31 January 2021 6,321 1 View

How to select substrate concentration to determine the inhibitor behavior as competitive or non-competitive?

I already calculated the IC50 value of the inhibitor against the protease enzyme. Now I need to find this inhibitor competitive non-competitive. I decided to perform the Lineweaver Burk plot, but...

21 January 2021 8,378 3 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Can TEV protease cleave between glutamine and methionine efficiently?

Based on this paper The P1 ' specificity of tobacco etch virus protease ,TEV protease can cleave between the Gln and several amino-acids (besides Gly/Ser) with acceptable efficiency in its...

26 November 2020 5,987 4 View

What is the best method to check interaction between a drug and a protein ?

Hi, I would like to know the best method to check the interaction between a proteine (cruzipain, cysteine protease) and a drug. It has been purposed to our project to evaluate protein-drug...

30 October 2020 7,209 3 View

Best method for immunostaining FLAG-tagged LRRK2 in rodent brain?

I have been carrying out IHC to stain FLAG in fresh-frozen rat brain using FLAG M2 (F1804) with ABC then DAB, but am unable to identify positive staining, and seeing a high background. I carried...

08 October 2020 8,986 2 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View

Protease activity assay?

Hello, I would like to get help! I want to measure the protease activity of pancrease powder. I prepared 10mg/ml stock solution from the pancrease powder and I used 100ul from that and I added...

22 June 2020 1,609 3 View

Why my protein has lower molecular weight that expected?

Hi, I am trying to make a kind of recombinant protein (Flag-tagged) from HEK293T cells. But when I check the cell lysate by WB, the molecular weight is lower that expected. The molecular weight...

09 June 2020 6,414 13 View