What is the best way or software to mark recombined stretches of a vegetable virus in refrence to published sequences of the same viruses ?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View

Any suitable Animal models to evaluate Mast cell stabilizers?

I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.

14 July 2024 779 0 View

What is the mathematical expressionof theoretical yield strength calculated considering solution strengthening in stainless steel 316L?

In the expression for solid solution strengthening model that has been widely used in the literature use a linear form consisting of strength coefficient, and concentration of elements in wt.%....

11 July 2024 4,476 3 View

How to insert cholesterol inside DPPC membrane by replacing same number of DPPC molecules in GROMACS?

I am trying to insert cholesterol inside the DPPC membrane by using gmx insert_molecules -replace. But number of DPPC molecules replaced is much higher than the number of cholesterol inserted?...

30 June 2024 4,160 2 View

How to synthesize Dichloro(p-cymene)ruthenium(II) dimer in a good yield?

Suggest some procedure where i can synthesize [Ru(p-cymene)Cl2]2 in a good yield.

23 June 2024 9,244 2 View

Analysis of microbial biomass for nutrient cycling?

It will be an honor, if anyone can suggest the standard methodology for analyzing nutrients(N,P,K) in soil microbial biomass

23 June 2024 3,850 4 View

Can i use polystyrene as a matrix in a hybrid COMPOSITE?

fiber powders will be used as a reinforcement.

23 June 2024 10,027 3 View

After expert validation of the research tool for data collection, what should be done first after piloting. Reliability analysis or factor analysis?

Which would be prefered first (factor analysis or reliability analysis of a Likert type scale)

19 June 2024 9,378 4 View

Which media will produce more biomass in saccharomyces cerevisiae?

Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...

18 June 2024 2,106 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?

27 July 2024 9,147 4 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Printing residues in an object in Pymol?

Hi! I am currently working on a protein where I have selected residues within 10A of its bound ligand in pymol. However, I can not find a way to print out what residues are contained in that group...

15 July 2024 1,727 1 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View