I have a problem of high bio burden (TAMC TYMC E choli etc) in some of my products. I have managed to reduce Yeast and Fungi with dry heating at 100 C but i am not able to reduce E .Choli . Please guide me any simple procedure to do so!
Any simple method of reducing Bio Burden?
You could ask Dr. Thomas McDonald at University of Nebraska Medical Center. I think he has a method for this.
Thankyou NORA for the information, I will surely ask Dr Thomas regarding this information.
can You please tell me that if my TAMC and TYMC has reduced to a very low limit , is there a possibility that Pathogens might have also been reduced , what i inferr is that all these bio burdens are inter related. i.e if one is reduced then the other also reduces.there might be some problem in my testing part also ( that i will check on my side).
Tom has a method to stop bacteria from replicating in some materials (perhaps in your process). If they do not replicate, it should reduce the amount of spores, etc from bacteria in your products. But he would know more.
You need to look for the root cause for high bioburden. Is it coming form environment / raw material? Are cleanroom procedures stringently followed? If E.coli is the problem, is it coming from your water? You need to confirm quality of your water purification and sterilization system also. Post-production reduction will require harsh treatments; if it is a serious problem, you may try low level (~5kGy) gamma irradiation if the product can sustain.
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