No title

09 October 2014 1,699 2 View

What is the best way of reducing bioburden in a finished powdered product?

I have a problem of high bio burden (TAMC TYMC E choli etc) in some of my products. I have managed to reduce Yeast and Fungi with dry heating at 100 C but i am not able to reduce E .Choli . Please...

06 July 2014 629 4 View

Is there any compound named potassium biochromate?

I have an analysis which involves the use of Potassium Bichromate but I am not able to find it in any chemical list. Are Potassium Dichromate and Potassium Bichromate the same thing or different?

08 September 2013 9,205 2 View

How can we use the value of A(1%, 1cm) in the calculation in an analysis by spectrophotometer?

I have a assay where absorbance has to be measured and in which the A% value is given and I want to find out the % of the material present. The method is according to the pharmacopeia . please guide.

08 September 2013 3,360 7 View

Can anyone suggest a simple method of determination of chloride in berberine tannate?

Please suggest a simple method of determination of chloride in berberine tannate. The method given in JP (Japanese Pharmacopoeia) is very complicated and is very confusing. Please suggest any...

07 August 2013 2,943 2 View

Reaction and manufacturing technique of Polyurethane foam using Polyol and MDI

Can anyone guide me about the mixing ratio of Polyol and MDI for the formation of Polyurethane foam(PUF) and Polyurethane coating liquid?

04 May 2013 9,707 1 View

No title

03 April 2013 1,721 2 View

Can any one guide me about designing of extraction plant by super Critical Carbondioxide

I have a project on designing of extraction plant by super critical carbon dioxide, what are the essential steps?

31 December 2012 7,665 2 View

Can anyone explain the use of Sodium Hexametaphosphate in Boilers?

What is the ratio in which Sodium Hexametaphosphate should be added in the boiler water to prevent scaling?

31 December 2012 854 3 View

Can anyone guide me with the simple assay methods for the different salts of berberine? Titration or a UV method is preferable.

Berberine

31 December 2012 9,041 0 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View