you can use the toxoDB that has multiple strains and search for genes and their sequences

http://toxodb.org/toxo/

get an overview over available database evidence.

probably stat with blasting the sequence to see what is out there.

I often download available data afterwards to align them myself to find subtle  differences or un-described transcripts.

make a comparison among the complete sequences  for y

You can find the original record of the gene and then the blast programs could use to acquire the complete sequence of target gene in your genome database.

Hello,

First you can download a gene sequences (from NCBI or Ensemble web site using Gene ID or gene name) specific to organism you are studying  (ex. Toxoplasma (http://www.ncbi.nlm.nih.gov/genome/genomes/30?), Human, Mouse, Rat, birds, amphibians etc.). Then look for the specific coding regions and non-coding regions of GRA6 gene. It also depend on your question, whether you are interested for sequencing only coding region or only non-coding region or both. Now you can save these sequences in your computer. Then you design the primer for each exon and intron (make sure you don't miss any region) and amplify them using the DNA from your organism of interest in a PCR reaction. After PCR collect your PCR product after running it on a agarose gel and elute it  or purify it for sequencing process. Then do the sequencing using your method of interest. Here you will use the sequencing primer. Once sequencing is done you can align the sequences for further analysis. You can use freely available software's for your sequence analysis (Ex. Finch TV) or other commercially available such as DNAStar (SeqAlign module)

I hope it helps.

Best

Ashok Singh

UW-Madison