In SDS-PAGE, if 2 entire lanes have more intense bands than the rest does this show increased protein expression?

I ran an SDS-PAGE gel of my total cell samples, each expressed in increasing concentrations of a co-factor to see whether this improves expression of the target protein. I coomassie stained the...

28 January 2020 1,772 3 View

Which cognitive biases are most useful for "justifying" teaching research methodology (social sciences)?

I am teaching an undergraduate course in research methodoloy (social sciences) for aspiring teachers and I usually start with explaining, why scientific methods are important to deal with...

24 May 2018 773 4 View

How to calculate standard errors for the model with measurement error in the explanatory variable?

17 January 2018 8,933 6 View

Can you recommend a good R package for VECM and cointegration?

Hi, can anyone recommend R package for cointegration and vector error correction model?. Ideally the package should be able to deal with both time series and panel data? Best regards KB

27 December 2017 5,264 13 View

Model for depression induction in rats?

25 August 2016 3,701 3 View

Does anyone have experience staining unstained molecular weight ladder prior to running gels?

I am currently running native gels. I need a molecular weight ladder between 1200 and 400 kDa. I only found one commercially but it is unstained. How can I stain it prior to running my gel?

13 August 2014 9,362 2 View

Does anybody work with breast cancer cell lines derived from rats?

Should it be the same principle as when you work with 4T1 cells and Balb/c mice?

30 January 2014 594 3 View

How does using literature to teach junior high reading instruction impact learning?

I am seeking articles that support and advocate for the use of the many types of literature available to learners today, with a focus on teaching junior high students. Thanks.

01 January 1970 4,112 1 View

Is there a single, reputable source listing the languages spoken w/in a country and the percentages of people residing in that country who speak them?

01 January 1970 2,328 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View