What is the best way for DNA isolation from older basidiomata (more than 6 months, dry herbarium items) of wood-decaying fungi? A reliable kit?

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Seeking innovative approach for "finding the impact of influencers in sentiment analysis in social networks" for thesis topic?

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Exploratory Factor Analysis: Order of Questions?

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BFI-44 scoring scale?

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29 January 2022 3,425 3 View

How to transform Navigator Pro FFR waves into numbers (FFT) using version 7.2.1?

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30 November 2021 7,009 0 View

Hierarchal regression analysis help?

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Kinase, Ligase and Dpnl in Q5 Site-Directed Mutagenesis (NEB) - What is the principle?

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10 August 2021 6,057 4 View

What can cause my MSCs culture media pH to raise slowly during a 14-days growth period?

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Do You have some reference on how the triple-helix (government/academia/industry) has been working on social disasters (e.g. outbreaks)?

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In SDS-PAGE, if 2 entire lanes have more intense bands than the rest does this show increased protein expression?

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Observational studies have to be registered on clinical trials?

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Is there a problem with my RNA pellet?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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RNA Extraction Using Hot Borate Method No Longer Working?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Nadia Jamil

Hi There

CTAB method is usually very good in such cases. for example

http://www.opsdiagnostics.com/notes/protocols/ctab_protocol_for_plants.htm

hope it helps.

Britta Boserup Thestrup

Hi Terezia

Whenever I have worked with older material I have allways isolated DNA with the CTAB method, but modified, so the concentration is 2 x CTAB.

Best Britta

Terezia Beck

Dear Nadia and Britta, thanks for your answers!

My pleasure

Thanks for your answer..