Ghannimah Alghuwainem

4 Questions 1 Answers 0 Followers

Questions related from Ghannimah Alghuwainem

RNA extraction from the bone - Any tips for good quality RNA?

Hello I am trying to extract RNA from the bone I have tried the Trizol method and I got a high yield of RNA with slightly low 260/280 ratio. when I measure the RNA integrity I got very bad...

06 September 2021 7,960 1 View

How much of cells needed to get good number of RNA ?

Hello I am growing MSCs cells in either 24 wells or 12 wells plate and then I need to lysate these cells for RNA extraction I ususally get RNA from 5 to 30s ng/ul Is there is any idea or tips...

27 January 2021 8,688 3 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

27 January 2021 9,372 5 View

How can I know the maximum number of seeding density of MSCs cell culture for a plate T175 ?

I am new in doing MSCs culture, i have a doubt about how can estimate the maximum number of the cells to be seeded for example, I am told to plat 4000 cells / well in 24 well plate but why I am...

01 January 1970 9,701 3 View