thank u so much Ms Danijela Markovic and Mr Shankhadeep Chakraborty regarding to worth information, PLz. if you have this article and insulin secretion assay in more details could you email me.
We routinely use INS-1 cells and find that these have a robust glucose-response. I believe MIN6 are a little fussy (although I haven't worked directly with them), although their glucose-responsiveness is strongly augmented when they are configured as 3D aggregates (pseudoislets).