I would like to know the best time after a pharmacological treatment. 5 min, 15 min, 30 min, 1 hour?
Seriously, there has to be way more detailes in this question, like a short method part.
Phosphorylation events happen rather quickly (within mins) but can also decline rapidly. That is why typically you would want to do a range of times such as the ones you have listed to see what works for your system. Also, most reviewers would expect to see moe that a single time point for data on phosphorylation for the main effect.
Times would be be different for cell cultures (5-10 min) slices (30-60min) and systemic injections (1 -2hr)
When you put an inductor like glucose --> insulin --> IR --> IRS --> PI3K --> Akt --> Pkc
--> GLUT translocation (In 2 hours from glucose intake recovers normal levels of glucose in blood).
--> inhibition GSK3 --> less inhibition GS --> glycogen (glucose -> glycogen)
GLUT and Gsk3 are before Akt activating. (In 1 hour plenty action, in 2 hours normal levels of glucose in blood, physiological), (GLUT make the entrance of glucose to cell).
You must look the Cmax of the drug (plasma concentration), and then 1 to 2 hours. I think. (Depends of the action of drug).
I made a draw about it time ago.
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