Anyone have experience with intracellluar Calcium measurement and staining with FLUO4-direct and imaging using florescence or Flow cytometry!

I need your help in finding a good protocol for Immunohistochemistry of brain sections for intracellular calcium in the neurons. I have the Fluo-4 direct Kit for measurement of intracellular...

03 April 2017 7,827 1 View

What is the most accurate way to quantify capillaries in a mouse brain section?

I did immunohistochemistry for brain capillaries and I am looking for an accurate way to quantify the number of capillaries and if they are affected by a certain treatment. Which software I should...

07 August 2016 4,636 6 View

Help needed in using ImageJ/ImageJFiji for the quantification and assessment of blood vessels and capillaries ?

07 August 2016 4,928 4 View

Aging and blood brain barrier dysfunction : What are the most significant findings to date?

I am trying to understand the relationship between the healthy aging process and the development of the blood brain barrier disruption which might lead to several neurodegenerative diseases such...

06 July 2016 2,354 8 View

Alzheimer's disease: What makes it that complicated to find a cure?

Since all the treatments that have been approved by the US Food and Drug Administration (FDA) for Alzheimer's. For example, cholinesterase inhibitors and memantine can help treat memory and...

01 February 2016 3,799 30 View

What is the best method of assessing the physical integrity and permeability of the blood brain barrier?

I am trying to find the best way to measure the blood brain barrier permeability Invivo (Mice),I tried the Evans blue dye with High molecular weight dextran but I'm still looking for a better...

31 December 2015 9,059 7 View

What is the best way to wash-out the mouse brain capillaries from blood after injection of a dye for Immuno-histochemistry?

I am trying to remove the blood from the brain capillaries after injection of a dye, I tried intracardial injection of Ice-cold heparinized PBS but I faced high resistance during the injection, so...

31 December 2015 8,381 4 View

Can anyone recommend a protocol to measure the Blood Brain Barrier permeability In-Vivo. other than Evans blue dye?

Hi, I am searching for a good protocol to measure the Blood brain barrier permeability in mice model without using the Evans blue dye.

09 October 2015 5,892 7 View

What is the best transfection kit and protocol for transfecting murine neuronal cells (Neuro2A) with APP to produce amyloid beta?

08 September 2015 447 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View