Sweilem Baseem Al Rihani

10 Questions 28 Answers 0 Followers

Questions related from Sweilem Baseem Al Rihani

Anyone have experience with intracellluar Calcium measurement and staining with FLUO4-direct and imaging using florescence or Flow cytometry!

I need your help in finding a good protocol for Immunohistochemistry of brain sections for intracellular calcium in the neurons. I have the Fluo-4 direct Kit for measurement of intracellular...

04 April 2017 7,902 1 View

What is the most accurate way to quantify capillaries in a mouse brain section?

I did immunohistochemistry for brain capillaries and I am looking for an accurate way to quantify the number of capillaries and if they are affected by a certain treatment. Which software I should...

08 August 2016 4,708 6 View

Help needed in using ImageJ/ImageJFiji for the quantification and assessment of blood vessels and capillaries ?

please I need your help in using ImageJ software for the quantification and assesment of brain blood capillaries in a different brain sections for immunohistochemistry for the evaluation of...

08 August 2016 5,016 4 View

Aging and blood brain barrier dysfunction : What are the most significant findings to date?

I am trying to understand the relationship between the healthy aging process and the development of the blood brain barrier disruption which might lead to several neurodegenerative diseases such...

07 July 2016 2,428 8 View

Alzheimer's disease: What makes it that complicated to find a cure?

Since all the treatments that have been approved by the US Food and Drug Administration (FDA) for Alzheimer's. For example, cholinesterase inhibitors and memantine can help treat memory and...

02 February 2016 3,873 30 View

What is the best way to wash-out the mouse brain capillaries from blood after injection of a dye for Immuno-histochemistry?

I am trying to remove the blood from the brain capillaries after injection of a dye, I tried intracardial injection of Ice-cold heparinized PBS but I faced high resistance during the injection, so...

01 January 2016 8,466 4 View

What is the best method of assessing the physical integrity and permeability of the blood brain barrier?

I am trying to find the best way to measure the blood brain barrier permeability Invivo (Mice),I tried the Evans blue dye with High molecular weight dextran but I'm still looking for a better...

01 January 2016 9,132 7 View

What is the best technique to remove Evans blue dye from the blood brain barrier vessels in mice?

In order to measure the Blood Brain barrier permeability I am trying to inject Evans blue dye and after six hours Ill take the mouse brain but I need to remove the Evans blue out of the blood...

12 December 2015 5,234 1 View

Can anyone recommend a protocol to measure the Blood Brain Barrier permeability In-Vivo. other than Evans blue dye?

Hi, I am searching for a good protocol to measure the Blood brain barrier permeability in mice model without using the Evans blue dye.

10 October 2015 5,997 7 View

What is the best transfection kit and protocol for transfecting murine neuronal cells (Neuro2A) with APP to produce amyloid beta?

Hi I am looking for a good protocol and kit for transfecting mice neuronal cells with the amyloid precursor protein APP in order to produce amyloid beta. I really appreciate your help and support.

09 September 2015 526 2 View