I am in problem with the protein estimation by BCA assay along with the protein carbonyl assay. Actually the recovery of protein is so poor (around 50%). What can I do now?
You might compare your protein determinations with N determinations according to Kjehldal or Dumas to decide which one is more right. Another question is do the carbonyl essay and the BCA measure the same things? If not, no wonder they give different results!