we would like to buy spectrophotometer for plant phyisology lab. what is your opinion about Thermo Scientific GENESYS Vis and UV-Vis 150 Spectrophotometers? or any other suggestion?
to measure chlorophyll, some enzyme, protein and ...
What do you mean by best?
- Accuracy over a wide concentration range?
- Number of samples in chamber? (single or multiple may affect the type of experiments you can successfully perform)
- Connection(s) to a PC or other recorders? What software is needed/included/or needs extra $.
- Ability to do time-based measurements? (for enzymatic reactions - how easy or hard will it be do do replicates)
- Ease of use over a long period? Ergonomics may be critical
- Cheapest? Do more features justify the extra cost?
- Smallest bench-top footprint? Few labs have room to install a whole new device without shuffling something around.
- Quality of after-sales service from vendor in your region?
If you have the opportunity, ask several vendors to bring in a test unit. Or visit other labs with similar (or alternative) models to the one you are considering. Try them out. What works for another lab may not be at all suitable for yours.
The best option is a Vis and UV-Vis MULTIPLATE Spectrophotometer.
I agree with Matthew, you need to ask what you (may) want to do with it. It may be, for instance, that you'd like to measure fluorescence
Dear Matthew Wheal Paul Milham thanks for answering.
Our budget is around 8000$. In our lab we need to measure chl, some enzyme, Protein, lycopene, proline, anthocyanin...... mostly we are working on plant nutrient and abiotic stress on vegetables.
it's not really important to connect to pc or no. multiple samples in chamber
easy to use.
Then it's simple: compare the wavelength range with what you may want to measure and purchase the instrument with the lowest stray light (highest 'linear' max absorbance) range. You will be able to it retrofit a flow cell to speed up measurements.
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