many research used water instead of DMSO to make serial dilution from fluconazole while other antifungal agent diluted with DMSO like Terbinafine or Griseofulvin
Dear Ali, you will find details on how to solubilize lipophilic antifungals in:
"Prediction of the solubility in lipidic solvent mixture: Investigation of the modeling approach and thermodynamic analysis of solubility" by Shruti V. Patel, Sarsvatkumar Patel, Eur J Pharm Sci. 2015 Sep 18;77:161-9.
The following work shows that there are no differences between one or another solvent (DMSO or water)
Abstract
A total of 145 clinical strains of Candida species were tested by the Clinical and Laboratory Standards Institute M27-A3 methodology to determine if replacing water with dimethyl sulfoxide as the solvent for fluconazole and flucytosine impacted the in vitro potency. No significant differences in MIC values were observed with either antifungal between the two solvents against any Candida species, and the essential agreement for each agent between the two solvents was greater than 99%.