What is proper injection solvent for drug insoluble in water?

Dear all, I use hydrophobic or insoluble drug for injection What is proper solvent that is low cytotoxicity ?

31 December 2016 2,659 4 View

Oxigen, double bonded to benzene, is carbonyl oxigen?

Dear all, Oxigen, double bonded to benzene, is carbonyl oxigen? (e.g. Phenoxide) Thank you for reading my question.

11 December 2016 9,389 0 View

Biomolecule and polymer have zetapotetial ?

09 October 2016 9,988 2 View

How to calculate pecent of injected dose per gram of organ ?

Dear all, Total injected dose : 100ug/100uL Gram of organ: 16mg Uptaked Drug concenration in organ: 1ug/mL How to calculate %ID/g ?

08 September 2016 9,559 2 View

How nanoparticles bind with thiolated compound ?

06 July 2016 1,485 1 View

Why use coupling reagent [ex) EDC/NHS] ?

Good afternoon :) I want to bind carboxylic acid-SAM layer with protein having amino group. In too many paper, coupling reagent is used for 'activation'. I know that carboxyl group react with...

04 May 2015 3,287 1 View

Is Osmium tetroxide necessary for SEM?

I want to make cell sample for measuring SEM but I don't have osmium tetroxide ( I heard it is expensive reagent...) I have to use osmium tetroxide? or negligible? Are there any other alternatives?

03 April 2015 5,190 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View