Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

When is it optimal to choose an OFAT (One factor at a time) design over DOE (design of experiment)?

As a current undergraduate student majoring in Microbiology who is still learning DOE (Design of experiment) on my own, I have heard and read that an OFAT design is considered the most inefficient...

29 January 2021 8,256 3 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Why is my blot transfer bad now?

I had been having beautiful blot transfers, but in the last few days I have ran into issues where there is high background staining and poor band resolution. The background staining is very...

23 December 2020 670 3 View

How/where to provide a dataset?

If someone has collected a large useful dataset that could be useful to social science research, gender research, a specific industry sector in business, and possibly business students or those...

05 October 2020 9,236 6 View

Does amelanotic B16-F10 cells give rise to black toumour nodles when injected in mice?

I am culturing B16-F10 cells in RPMI as these cells tend to die when cultured in DMEM high glucose. When cultured in RPMI, cells are amelanotic. If I inject these amelanotic cells in vivo (i.v.),...

02 September 2020 3,524 1 View

Why do 293T cells starts dying after lentiviral transfection?

I have a lentiviral transfection protocol on 293T cells that has been working for years, but for the past few months, my 293T cells starts de-attaching from the plate. I finally figured out it was...

16 August 2020 6,647 3 View

Will making a hydride acceptor more electrophilic speed up hydride transfer?

I have been searching the literature about this and I have not found an answer. If the atom that accepts a hydride is made more electrophilic, can this speed up the rate of hydride transfer?

29 July 2020 9,944 1 View

Can the energy displayed in Gaussian Calculation Summary be used to compare the stability between molecules?

I want to compare the stability of molecules that have the same number of atoms. I optimized these structures and calculated their vibrational frequencies. Gaussian09 gives a Gaussian Calculation...

18 July 2020 5,243 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How to dissolve querceitin in HEPES?

I am trying to do the isothermal titration calorimetry study of quercetin. I want to reach a final concentration of 5 mM quercetin in HEPES or MES buffer with

15 February 2021 2,159 3 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

My research is entitled "BATTERY OPERATED TWO COLORED LIGHT LAMP USING DIMMER SWITCH BRIGHTNESS CONTROLLER" can anyone suggest me related studies?

I badly need it,please

07 January 2021 8,682 3 View

DNA pol quantification?

Currently, I'm working on a DNA pol activity test, one of the variables is DNA pol concentration in the PCR/LAMP assay. For LAMP I found a clear protocol for the DNA pol in weights. However, for...

01 January 2021 9,869 3 View

The saturation point does not reach in my ITC. Does this indicate that the interaction is weak or there is no interaction?

21 December 2020 9,407 8 View

How can I solve my False-Positive issues with the SARS-CoV-2 RPA amplification ?

Here, I want to ask about my issue with the SARS-CoV-2 RPA amplification False-Positive results. I tried two different sets of primers. The first sets of primers are 29 nt producing 200 bp...

20 December 2020 2,091 3 View

Can LAMP distinguish the species from different genus/family?

I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...

07 December 2020 5,630 3 View

Smering in Pcr Amplification ?

I am facing a problem regarding smearing in PCR amplification since two months. But, before six months the results was there using the same primers at 67.2-degree celsius annealing temperature, 5%...

23 November 2020 4,394 5 View