What is the best reference gene in serum for normalizing miRNAs relative quantification by qRT-PCR ?

More Nasim Vatandoust's questions See All

Can I rely on target prediction data bases without any experimental evidence to design a study of quantification miRNA in serum ?

I know miRNA that targeted a gene in rats and has been proven experimentally. The hsa- miRNA is identical to rno-miRNA, so I decided to quantify this miRNA in a patient's serum instead of a cell...

10 November 2012 8,764 0 View

References for microRNA quantitative PCR in serum to compare patient and control groups?

Any recommendations for references of microRNA normalization q-rt pcr in serum samples?

03 April 2012 3,335 0 View

Can I rely on an microRNA gene target published in an article but not included in target database?

When a gene is claimed as a microRNA target in a paper and after experimental processes, can i rely on it to continue my research?

02 March 2012 8,439 10 View

hi all researcher can any one suggest me the way to knockdown endogenous Mirna?

i want to knockdown endogenous Mirna in a cell line by an antagomir but i don not know about it and i need some one that is experienced help me

31 December 2011 8,369 13 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Fatemeh Mirzadeh

it is hard to say which one is the best, different papers suggest different factors miR-16 and let-7a are also reported. you must compare the paper experimentt condition with yours.

Jo Vandesompele

We recommend to use the modified global mean expression level of all microRNAs as best normalizer (see D'haene et al., Methods Mol Biol. 2012;822:261-72)

http://www.biogazelle.com/papers/Dhaene_2011.pdf

Nayan Jyoti Sarma

miR-16 and miR-93 as described in "Identification of suitable reference genes for qPCR analysis of serum microRNA in gastric cancer patients." by Song et al

Nasim, take a look at this: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2327352