What is the best reagent to induce necroptosis in HEK-293 cell line?

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How to calculate the times a random experiment needs to be conducted (diff samples of same population) for generalisability?

any scientific method?

20 January 2024 7,632 0 View

Is it acceptable to add more conclusions in a conference paper that were not discussed due to shortage of space?

Hi, My research project had numerous results and because the conference paper is usually just 5-6 pages long, I could not discuss all my results in it. However, is it still possible to mention...

04 May 2020 721 5 View

Missing atoms from CONTCAR file after the simulation run for geometry optimization. Why??

I am trying to run geometry optimization of a catalyst structure and a reactant on its surface. AFter the simulation, I observed 10 atoms (2 Oxygen and 8 hydrogen atoms) from the reactant molecule...

23 April 2020 2,959 0 View

How long will it take to digest the food in the absence of digestive enzymes in our body?

We all know that digestive enzymes speed up the chemical reaction and helps in food digestion. I was wondering if in general it takes 6 h to digest a food with the help of digestive enzymes then...

04 October 2019 5,655 3 View

Can anyone suggest me a better coating reagent other than Poly-L-Lysine for the adhesion of HEK-293T cells?

I recently coated the 96 well plates with 0.1 % w/v (1:10) dilution of Poly-L-Lysine for seeding the HEK-293T cells. However, I found that the attachment of the cells to the surface is not...

24 May 2019 476 2 View

How to perform cell based assay in 96-well plate using HEK-293 cells as the cells shows poor adhesion and comes off quicking ?

I have to perform fluorescence based assays in 96-well plate using HEK-293 cells. The experimental procedure involves transfection in cells and then treatment with drugs for testing drug efficacy....

01 May 2019 3,738 9 View

Does all enzymes are synthesised by ribosomes in zymogen form or are there expections when enzymes are synthesized in active form?

Enzymes are synthesised as zymogens and then make a transition to active form when required, is this true for every enzyme or are there any exceptions to that?

17 March 2019 4,480 3 View

Why am I seeing expression all over the cell while expressing EGFP-N1 plasmid in MCF-7 cells?

I have expressed EGFP-N1 plasmid (1ug) in MCF-7 cells. Upon taking the confocal images under 40x oil I have seen the expression all over the cell? What is the possible reason for that? Can anyone...

27 February 2019 6,380 6 View

Is it possible to obtain a dimer in SDS-PAGE reducing gel?

I have purified a monomeric EGFP- His protein with a molecular weight of 30 kDa. But, instead of 30 kDa I am getting a thick band of 60 kDa in SDS-PAGE reducing gel. what is the possible reason...

30 January 2019 5,899 2 View

Why the Ni-NTA purified His-tagged EGFP protein is coming at 60 kDa instead of 30kDa on SDS-PAGE gel?

I have purified a EGFP-His tagged protein which was there in pET15b vector using Ni-NTA column. The concentration obtained was way too high around 5mg/ml. Before the running the SDS-PAGE the...

30 January 2019 7,380 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Is it possible to dock more than one drug in the same active site or pocket of a protein to study synergistic/antagonistic/additive properties?

I wanted to know whether we can observe the synergistic/antagonistic/additive properties of combinations or mixtures of compounds through docking analysis. But during docking preparation any...

28 July 2024 7,413 6 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View