What is the best quenching solution for pichia pastoris in order to quench the yeast metabolism immediately?

Mohammad Amin Boojari @Mohammad-Amin-Boojari

16 January 2019 1 10K Report

I want to extract total RNA from pichia pastoris to evaluate the yeast transcriptome using RNA-Seq.

Is that absolutely necessary? look at this ref they simply extracted RNA using RNAeasy kit from treated and untreated condition.

Article Comparative genomics and transcriptomics of Pichia pastoris

Best wishes,

Hanna

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Science method

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