What is the best quality, most economic (per virus genome) deep sequencing service in Europe?

More Christopher H. Logue's questions See All

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Why do irreversible inhibitors only affect Vmax and not Km?

So I've been wondering why irreversible inhibitors only affect maximal velocity and not Km? Is there a...

19 January 2021 2,509 1 View

In the Venusian atmosphere why doesn't Nitrogen which is much lighter Carbon Dioxide float to the upper regions?

Carbon dioxide on Earth weighs 44 g/mol and has a density of around 1.98 kg/m3, about 1.53 times that of air. Nitrogen gas weighs 28 g/mol and has a density of 1.2506 g/L. During limnic eruptions...

15 January 2021 8,733 4 View

What type of cellular structures are these likely to be?

I have immunohistochemistry slides of human subcutaneous fat biopsy, with skin attached. Most of the structures are self-evident - skin, adipocytes, microvessels in transverse section (revealed by...

03 December 2020 4,238 7 View

Mata-analysis question: How to deal with multiple outcome scales used within individual studies?

Dear all, thanks for the help in advance. I am performing a met-analysis. Most of the studies included report the same outcome using multiple scales e.g. MMSE or CDRS. What is the best way of...

30 October 2020 8,629 5 View

Glucose dehydrogenase interference with glucokinase assay?

Hello all, I'm currently working with undergraduate student-researchers (there are no graduate students at my university) to purify and characterize the drug targetability of glucokinase from pig...

25 October 2020 3,553 7 View

What is the minimum number of timepoints for markov models?

For those who frequently work in markov models, I am interested in knowing the minimum number of time points/observations to get stable estimates of parameters. I have some follow ups, too:...

24 September 2020 9,659 3 View

How best to address statistical differences when there is no difference in the independent variable?

If an experiment includes, say, two groups and Condition 1 is identical between groups but Condition 2 differs between groups, what is the best course of action if one finds a significant...

17 September 2020 9,731 7 View

Can you re-use ethidium bromide post-stain solution more than once, or does ethidium bromide need to be added fresh each time?

When post-staining agarose gels in an ethidium bromide / water bath for 10 minutes, my labmate says she can re-use the post-stain solution 2-3 times before she has to add more ethidium bromide (at...

04 September 2020 680 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Andrey A. Yurchenko

We had our best experience with the Macrogen company.

Christopher H. Logue

Thanks, Andrey. how much was it a read, how big a genome were you sequencing and how deep were you reading?

Around 36Gb of data cost €2500, but it was about 8 months ago. Now they have a permission to use Illumina Hiseq X10 for non-human genomes, so I expect that this price should be much lower.