Most of the insects are rich in protein. Hence, I would suggest you first start with the well known BIURET method. If you find that still the proteins are higher than expected,then you may do the total nitrogen content using MicroKjeldhal method and then convert it to protein using some of the factors of conversion found in literature. If you feel that your insects are low in protein then go by the Lowery's method. Remember if the protein content is high and you have to dilute your protein solution several folds for estimation (say by Lowery's method)then errors will creep in and you might not even notice it till it is very late.
Dear Priya
This paper can help you
You an read it : Article Extraction and characterisation of protein fractions from fi...
The method for protein extraction from whole insect body will be depends on the fate of that sample, so, it would be more specific if you add this!
It would be better to add the target of your desired proteins like if you want to use for 2D separation, western blott, LC-MS/MS or any other
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