How can I apply a thermo-mechanical load in hypermesh?

I want to determine the stresses for a laminated composite plate under thermo-mechanical load.

24 May 2019 9,180 4 View

Can I get MATLAB code for vibrational analysis of composite plate?

Iam working on vibrational analysis of laminated composite plate using MATLAB.

28 October 2018 7,641 3 View

A Question About Discretization of the Population Balance Equation...?

Hello my friends, Since there is not a direct reference for discretization of source terms of PBE with the discrete method in Ansys FLUENT Help 17, I thought it would be a good idea to ask...

12 May 2018 381 1 View

How we can determine individual sum of squares for independent variables (factors) in the central composite design?

I am trying to solve central composite design ANOVA table manually. It has total 30 number of runs which includes 16 factorial runs,8 axial runs and 6 center runs. so, far using regression method...

23 April 2018 2,103 1 View

Why carcinoma cell lines (e.g. HepG2, Caco-2, RAW) are used in invitro cytotoxicity experiments?

HepG2 is considered as a model system for various cytotoxicity experiments according to many studies. Won't it produce wrong results if carcinoma cells have been used for such studies as such...

21 August 2017 5,310 7 View

Is there any standard criterion to select optimum number of sequence candidates for establishing a phylogeny tree in bacteria using MEGA?

for example, in case of 16s rRNA nBLAST results, I have almost 80 similar sequences with % similarity ranging from 99% and going down till 94% ?

16 March 2017 4,772 2 View

Nusselt Number corelation for hot liquid flow through inner annulus of pipe in case of Laminar flow.

I encountered a problem in my project work on heat exchangers where to find heat transfer coefficients on the defined conditions the Reynolds number came to be 1753.8 while the Prandtl number is...

08 March 2017 4,652 3 View

Can a substitution mutation in an exon result in splice site changes?

I have found a novel mutation in clotting factor 2 gene in an exon (midway through the exon), however, on checking with mutation tester it is showing that the mutation may result in splice site...

01 March 2017 8,737 3 View

How can I build annotated corpus for identification of uncertainty in twitter data ?

20 September 2015 5,567 7 View

How to generate a daily global composite from the ascending and descending swaths of soil moisture retrieved data

If any material is available for some method on how to generate this global composite, then please send me the link to it too

16 September 2013 7,879 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View