The temperature, crystallization buffer and incubation time etc?
I am working on a 26kDa heat shock protein. I understand that to induce a heat shock protein is by heat shock, but what is the best way to induce, express and purify a heat shock protein in a...
07 August 2019 7,936 1 View
I tried to read journals but the explanations are too complicated
09 October 2017 232 3 View
I mean, after doing affinity chromatography with nickel beads, we do SDS-PAGE, then why do we need to proceed to ion-exchange chromatography? Then after that, another SDS-PAGE, then we proceed to...
09 October 2017 8,958 8 View
I mean, after doing affinity chromatography with nickel beads, we do SDS-PAGE, then why do we need to proceed to ion-exchange chromatography? Then another SDS-PAGE, then we proceed to size...
09 October 2017 8,662 7 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
