Shahrizal Umar

5 Questions 9 Answers 0 Followers

Questions related from Shahrizal Umar

What is the best way to induce and express a heat shock protein?

I am working on a 26kDa heat shock protein. I understand that to induce a heat shock protein is by heat shock, but what is the best way to induce, express and purify a heat shock protein in a...

08 August 2019 7,941 1 View

What is the best optimization to crystallize periplasmic protein?

The temperature, crystallization buffer and incubation time etc?

11 November 2017 5,457 0 View

I'm quite new in the field of protein purification. I want to ask how to introduce TEV-protease cleavage site and GFP-His tag into genes?

I tried to read journals but the explanations are too complicated

10 October 2017 239 3 View

I'm new in the field of protein purification. I just want to ask, why is protein purification a multistep process? Can't it be just one single step?

I mean, after doing affinity chromatography with nickel beads, we do SDS-PAGE, then why do we need to proceed to ion-exchange chromatography? Then after that, another SDS-PAGE, then we proceed to...

10 October 2017 8,960 8 View

I'm new in the field of protein purification. I just want to ask, why is protein purification a multistep process? Can't it be just one single step?

I mean, after doing affinity chromatography with nickel beads, we do SDS-PAGE, then why do we need to proceed to ion-exchange chromatography? Then another SDS-PAGE, then we proceed to size...

10 October 2017 8,663 7 View