There are different methodologies to digest plant material for heavy metal analysis; for example, they use 1) nitric acid, 2) nitric acid + hydrogen peroxide, 3) hydrochloric acid, among others.
The best method for extraction of heavy metals namely Zn, Cu, Fe, Mn, Ni, Cd, Pb etc from plant sample is wet digestion with di acid mixture (HNO3: HClO4 ::3:1).
Thank you very much Muhammad Amjad for your reply. It was the answer that I was afraid of because of the risks involved in working with this mixture of acids.
Maybe i could help youhttps://yadda.icm.edu.pl/yadda/element/bwmeta1.element.baztech-d8cb0c6a-46c0-4968-aeec-1c8b6c144540
Hi Beatriz Pernia,
You are correct. You need a HClO4 certified fume cupboard for working with this mixture. My students use only the Honc HNO3 for powdered plant tissue samples. Please refer to my publication lists related to HM. we measure around 95% recovery by using this methodology.
"For each plant biomass sample, 0.1 g of dried and ground material was digested (at 120 degree C for 2 hours) with conc HNO3 (10 mL) and diluted to 25 mL with de-ionized water....."
- Badges
- Science topic
- Similar topics
- Agricultural Science
- Agronomy
- Crop Science
- Crop