Hi i would like to profile amino acid using uv hplc. Is it possible to do so without derivitisation. If no what is the best yet reliable methode to do the amino acid derivatisation?
a convenient way to analyze amino acids in biological samples is via HPLC on a C18 column with automatized precolumn derivatization using OPA and FMOC and fluorometric detection (FLD):
Article In vivo quantification of intracellular amino acids and inte...
Thank you for you answer, but does it mean that only OPA and FMOC derivitization can be only be measured by fluorescence detectio? Cause I'm still confuse about this. Can OPA and FMOC be be measured under UV detection? Thanks again for your reply.