SSF helps you to produce cellulase or any enzyme in large concentration, the main advantage but as Mr. Ved Prakash suggested it is difficult to engineer SSF rather than SMF. If you are targeting just production in your studies, then go for SSF just design the experiment using RSM or one factor at a time (old method). It depends on what are you targeting in your studies.

Thank you Mr. Ved Prakash & Mr. Ankit Sudhir. Your answer helps me a lot to decide which method is more preferable. Currently, for the beginning of my research work, i've produce the cellulase enzyme using SMF method instead of SSF. SSF is a new field for me and i never tried it before. Based on comment that i received from both of you, i will choose SMF since in future  i will go for a scaling up process to produce this enzyme in a large amount. 

I have gone through both process but i observed solid state fermentation medium is best one.

Based on my experience, it depends on the feedstock you use. If the feedstock from cellulosic material is better using SSF. If non cellulosic material better using SMF, such as from mollases. Fungi is aerobic microorganism, need more aeration to get better cellulase yield. So the system better use SSF for large scale production because well controlled

Thank you Dr. Zahid Anwar and Dr. Maizirwan Mel for your response to my question. Based on the answer received from both of you and also Mr. Ved Prakash and Mr. Anjit Sudhir, it is the best for me to study both method since different fungi have different properties. Maybe some of the fungi produce more cellulase in SSF and maybe some of it not. In my research i'm using cellulosic material as a feedstock. Dr. Maizirwan, you did mention that SSF produce better cellulase yield. May I know how much in comparison to the cellulase using SMF method. Thank you.

Ok, that's nice it is better to compare both type of fermentation. But you should planned your research according to industrial point of view.

Thank you Dr. Zahid. I agreed with you. My research plan should in-line with industrial needs. Because i'm targeting to produce enzyme for industrial application. 

I also suggest SSF in the form of a membrane bioreactor (MBR) for higher productivity. A brilliant example of an MBR system for your application is the membrane gradostat reactor. This reactor is relatively easy to assemble, operare, 

SSF using raw materials (which are also considered as agrowastes) is the most preferred method for cellulase production if the idea is to produce it for industrial application. Now a days it is the most preffered choice for enzyme production. Also cellulose containing raw materials are easily available at lower costs. So it will also reduce the cost factor.

My research area is also related to this topic. Currently SSF is known to produce high production of cellulase compared to SMF. The fungi itself ubiquitously and naturally exist and grow on the solid substrate in the environment. SSF mimic the natural habitat of the fungus. This advantages contribute to the high production of cellulase enzyme by the fungus.

But yet still depends on what kind of fungi species that you going to use.

I agreed with Dr . Maizirwan , solid state fermentation (SSF) is the best method for cellullase production from cellulosic material , rice straw is better cellulosic substrate for cellulase production.

May I ask, do you know how to prepare the inoculum using spore suspension? Currently I am working on SSF and fungi that I use are Aspergillus niger & Aspergillus flavus. Tq in advance :)

Solid State Fermentation simulates the natural way to produce cellulase by fungus. It's the best way to produce cellulase.However, its very very important the kind of the substrate to the production. The substrate should be rich in cellulose and other important thing is the pre-treatment of that substrate ( acidic hidrolysis, high pressure) can help to increase the disponibility of the cellulose from lignocellulosic biomass.

As we know both SSF and SmF are applicable for cellulase production by any fungi. But if there is a question about the cost then the best one is SSF. Fungi need much space therefore they will feel like their home in case of SSF. I suggest to go for SSF if the cellulase producing organism is Fungus.

All the best. 

-AKM