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Questions related from Angel Josue Arteaga
I'm working with Cladosporium cladosporioides, and I need to do an analysis by SEM to see the morphology characteristics and interaction with linocellulosic substrate. Anyone have a protocol for...
11 November 2013 7,889 2 View
I have a crude extract of enzymes produced by Solid State Fermentation, but the microorganism produces a black/ brown dye. I already tried to different methods to remove dye from extracts, like...
07 July 2013 5,441 3 View
We isolated one fungi from the soil, and from its morphology we concluded it must be a Cladosporium genera ( a filamentous fungi , a mold ). I've revised different methods, but I dont know what...
07 July 2013 2,926 10 View
I'm trying to separate cellulases from a crude extract, obtained from a Solid State fermentation of a Filamentous fungi with lignocellulosic waste. In a Zymogram we confirm the Molecular weight of...
07 July 2013 4,194 2 View
I am working with production of cellulolytic enzymes from fungus ( such as Aspergillus sp) and I want to perform Endoglucanase, Exoglucanase and β-Glucosidase Zymograms. I didn't have problems...
05 May 2013 2,601 6 View
I am trying to determine the activity of the enzyme β-Glucosidase, isolated from A. niger and other fungi, produced by solid state fermentation. The problem is that the absorbance is very high (A=...
04 April 2013 5,346 32 View
I am working in the production of cellulase from fungus (molds) like Aspergillus niger, but I have problems with the precipitation with ammonium sulfate. I don't see any precipitation in the...
04 April 2013 5,400 19 View