It depends on the consistency of the sample and intended parasite detection. 10% formalin works fine in most scenarios. This is cheaper and easier method for preservation. But I would recommend try at least 2 methods for preservation of single sample. For second method you can choose using MIF or PVA or SAF or any commercial kits. I personally prefer PVA. And if you are intending to testing antigen only then you can simply refrigerate the sample.
This depends on how you intend to identify the parasite.
E.g., in my field, the majority of parasite infections are identified by real-time PCR because of its sensitivity and specificity. However, formalin will cross-link and degrade the nucleic acids so that any recovered nucleic acids are fragmented. So, for real-time PCR analysis, feces should be stored frozen.
But freeze-thaws will damage the sample for detection of parasites by traditional methods, including direct smears, flotation, and sedimentation (for some organisms), and histopathology.
If you are not sure what analyses will be required, refrigeration allows you to keep your options open, since it keeps the sample acceptable for multiple diagnostic platforms.
Dear Sir/Madam,
See this link may be useful for you.
CDC - DPDx - Diagnostic Procedures - Stool Specimens
www.cdc.gov › ... › Diagnostic Procedures › Stool Specimens
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14 nov. 2016 - Fresh stool should be examined, processed, or preserved ... are not available; these specimens are suitable for antigen testing only. ... them adhere to slides); Preserved samples remain stable for several months ... For additional information on stool collection, call the Division of ParasiticDiseases at (404) ...
Best regards.
Nouhoum
Hi
It's depending upon the method of diagnosis, if you use the classical method you can preserve the fecal sample in 10% formal saline also it's useful in case of direct ELISA while in case of molecular methods you should preserve the sample in 70% ethyle alcohol or in deep freezing.
HI dear
the best method depend on parasite as fecal methods as flotation method
and direct E LIZA
molecular as PCR , rtPCR , RFLEP PCR ,....
hi
Preserve the specimen as soon as possible. If using a commercial collection kit, follow the kit's instructions. If kits are not available, the specimen should be divided and stored in two different preservatives, 10% formalin and PVA (polyvinyl-alcohol), using suitable containers. Add one volume of the stool specimen to three volumes of the preservative.
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